Thank you Bernard! Your suggested targets seem really good for the purpose. Since I made the stock solution for AO staining I didn't try to stain anything yet. So maybe it's time to start!
Both subjects seem cool and I will make some tests on them and update this post with the results!

I don't have a blog yet because I don't have much time to write every project down. I'm also working on laser microdissection and on my new SEM and plasma sputter. Maybe in the future I will put everything on a blog!

Thank you!
I don't have the means to measure the actual thickness of the slices. in theory it should be close to the objective depth of focus.
I know Sphagnum isn't the best choice, but I didn't have anything better to test! I should look for some cyanobacteria or maybe I will just use some higher ...

Hello!

Sorry but I can't speak French!

I thought to share my SIM (structured illumination microscope) project on my Zeiss Universal on this interesting forum.

Structured illumination allows to record confocal-like images of fluorescent samples. Due to the slow acquisition rate of my system (10 ...

Thank you Pierre,
the pneumatic micromanipulator is a "Micro-manipulator de Fonbrune" which I bought on Ebay for around 150€. It arrived with the frontal bellow of the probe moving unit broken, because of poor packaging. I had to fix that with soldering and structural glue and of course I asked for ...

sciroccoblow a écrit :Hi,

Verry beautiful work, and what a terrible microscope !

You've no problem to drive the prior stage?

Thank you. I can drive the motor at very low speed, but not that smooth as it is supposed to be with an original controller. Considering that I bought it for 100€ it's fair enough!

Thanks to all for your welcome!

Yes Patrice, sadly, I know him and his ego...... He is not well seen in our italian community for the reason you probably all know

Merci André,
j'espère parler bientôt avec Dominique!

Merci Yves!

Salut à tous!

Je suis désolé, mais je ne parle pas français!

So I need to speak english...!

My name is Franco, I'm a student of Natural sciences at the University of Florence, Italy, and I've been into microscopy for 2 years. I have started with my first scope for studying bryophytes (an old ...